Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with mouse CD272 antibody at 1/500 dilution (1 μg) / (right panel) compared with an Amenian hamster IgG Isotype Control / (left panel). Goat Anti- Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Alexa Fluor® 647 antibody separately. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo anti-Mouse CD272 Recombinant mAb
Invivo anti-Mouse CD272 Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD272 |
| Synonyms | B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; Btla |
| Location | Cell membrane |
| Accession | Q7TSA3 |
| Clone Number | S-R709 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM, Functional assays |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, Contains no stabilizers or preservatives |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
CD272 (BTLA) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
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