WB result of Invivo Anti-Mouse CD25 (IL-2Rα) Recombinant mAb
Primary antibody: Invivo Anti-Mouse CD25 (IL-2Rα) Recombinant mAb at 1/1000 dilution
Lane 1: IL-2R alpha/CD25 His Tag Protein, Human 1 µg
Lane 2: IL-2R alpha/CD25 His Tag Protein, Mouse 1 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 40-55 kDa
Invivo Anti-Mouse CD25 (IL-2Rα) Recombinant mAb
Invivo Anti-Mouse CD25 (IL-2Rα) Recombinant mAb
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$120 USD
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$120 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra |
| Location | Membrane |
| Accession | P01590 |
| Clone Number | S-3558-1 |
| Antibody Type | Recombinant mAb |
| Application | ELISA, WB, in vivo regulatory T cell depletion |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
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Western Blot
