Flow cytometric analysis of Mouse CD19 expression on C57BL/6 mouse splenocytes. Cells were labeled with either Rat IgG2a, κ Isotype Control (black line histogram) or SDT Rat Anti-Mouse CD19 antibody (red line histogram) at 1/500 dilution (1 μg). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo anti-Mouse CD19 Recombinant mAb
Invivo anti-Mouse CD19 Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD19 |
| Synonyms | B-lymphocyte antigen CD19; Differentiation antigen CD19; Cd19 |
| Location | Cell membrane |
| Accession | P25918 |
| Clone Number | 1D3 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2a, κ |
| Isotype Control | Invivo rat IgG2a isotype control, anti-trinitrophenol |
| Application | FCM, in vivo CD19 neutralization |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
CD19 is a 95 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is expressed on the surface of B lymphocytes and follicular dendritic cells. It plays a crucial role in B cell activation and humoral immune responses by forming a complex with CD21, CD81, and CD225. This complex reduces the threshold for B cell receptor (BCR) activation, enhancing downstream signaling pathways and facilitating B cell responses to antigens. CD19 is also a biomarker for B cell development and lymphoma diagnosis, and it is targeted in immunotherapies for diseases like leukemia.
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