Flow cytometric analysis of BALB/c mouse bone marrow labeled with Anti-Mouse CD172a antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG1, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Alexa Fluor® 647 antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo Anti-Mouse CD172a (SIRPα) Recombinant mAb
Invivo Anti-Mouse CD172a (SIRPα) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD172a (SIRPα) |
| Synonyms | Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs (Bit); CD172 antigen-like family member A; Inhibitory receptor SHPS-1; MyD-1 antigen; Signal-regulatory protein alpha-1 (Sirp-alpha-1; mSIRP-alpha1); p84; Bit; Myd1; Ptpns1; Shps1; Sirp; Sirpa |
| Location | Membrane |
| Accession | P97797 |
| Clone Number | S-R713 |
| Antibody Type | Rat mAb |
| Isotype Control | S0B7122 |
| Application | FCM, in vivo blocking, in vitro blocking |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse bone marrow |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
CD172a, also known as SIRP alpha (signal-regulatory protein alpha), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on cells of myeloid origin, including monocytes, macrophages, dendritic cells, and granulocytes, but not on T and B lymphocytes. SIRP alpha plays a crucial role in immune regulation by interacting with its ligand, CD47, which is widely expressed on various cell types, including cancer cells. This interaction transmits a "do not eat me" signal to prevent phagocytosis of healthy cells and is involved in processes like dendritic cell-mediated T cell activation and neutrophil migration. Additionally, SIRP alpha has regulatory effects on cellular responses induced by growth factors, insulin, and oncogenes, and it is implicated in both physiological and pathological processes, such as cancer progression.
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