Flow cytometric analysis of C57BL/6 mouse splenocytes treated 48h with 2.5 μg/ml Concanavalin A (right panel) or untreated (left panel), labeled with CD134 at 1/500 dilution (1 μg). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo anti-Mouse CD134 Recombinant mAb
Invivo anti-Mouse CD134 Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD134 |
| Synonyms | Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; Ox40; Txgp1; Tnfrsf4 |
| Accession | P47741 |
| Clone Number | OX-86 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG1,k |
| Isotype Control | Invivo rat IgG1 Isotype control, anti-trinitrophenol |
| Application | FCM, in vivo neutralization, in vitro cell specific stimulation/activation |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes treated 48h with 2.5 μg/ml Concanavalin A |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
CD134, also known as TNFRSF4 or OX40, is a member of the tumor necrosis factor receptor superfamily. It is a co-stimulatory molecule primarily expressed on activated CD4+ and CD8+ T cells, as well as other immune cells such as natural killer (NK) cells and dendritic cells. Structurally, CD134 contains four TNFR-Cys repeats and interacts with its ligand, OX40L (TNFSF4), to activate downstream signaling pathways, including the NF-κB pathway through adaptor proteins like TRAF2 and TRAF5. This interaction promotes T cell proliferation, cytokine production, and enhances the survival of CD8+ T cells, playing a crucial role in immune responses. Additionally, CD134 has been implicated in various diseases, including cancer and autoimmune conditions, making it a target for immunotherapy and other therapeutic interventions.
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