Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse BTLA (CD272) antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Rat IgG1 Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Pacific Blue™ Antibody separately.
Invivo Anti-Mouse BTLA (CD272) Recombinant mAb
Invivo Anti-Mouse BTLA (CD272) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | BTLA (CD272) |
| Synonyms | B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; Btla |
| Location | Cell membrane |
| Accession | Q7TSA3 |
| Clone Number | S-3831 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | FCM, in vivo BTLA stimulation, in vivo BTLA blockade |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
BTLA (CD272) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
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