Flow cytometric analysis of human peripheral blood cells labeled with Anti-Human VISTA antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo Anti-Human VISTA Recombinant mAb
Invivo Anti-Human VISTA Recombinant mAb
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$175 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | VISTA |
| Synonyms | V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; Stress-induced secreted protein-1 (Sisp-1); V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptorImported; C10orf54; SISP1; VISTA; VSIR |
| Location | Cell membrane |
| Accession | Q9H7M9 |
| Clone Number | S-5526 |
| Antibody Type | Mouse mAb |
| Isotype | Mouse IgG1, κ |
| Isotype Control | S0B0760 |
| Application | FCM, in vivo blocking, in vitro blocking |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Hu |
Background
VISTA (V-domain Ig suppressor of T-cell activation), also known as B7-H5, PD-1H, C10orf54, Dies1, Gi24 or SISP1, is a type I transmembrane immune-checkpoint protein of the B7-CD28 family that is highly expressed on myeloid and granulocytic cells and at lower levels on CD4+ and CD8+ T cells; it functions both as an inhibitory receptor and as a ligand on antigen-presenting cells to suppress T-cell activation, maintain peripheral tolerance, modulate innate and adaptive anti-tumour immunity, and may additionally regulate embryonic stem-cell differentiation via BMP4 inhibition and stimulate MMP14-mediated MMP2 activation.
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