Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labelling Human CD25 (IL-2Rα) antibody at 1/250 dilution (2 μg) / (Right panel) compared with a Mouse IgG2a Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.Then cells were stained with CD4 - Brilliant Violet 421™ Antibody separately.
Invivo Anti-Human CD25 (IL-2Rα) Recombinant mAb
Invivo Anti-Human CD25 (IL-2Rα) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA |
| Location | Membrane |
| Accession | P01589 |
| Clone Number | S-3707 |
| Antibody Type | Recombinant mAb |
| Application | ELISA, FCM, in vivo regulatory T cell depletion in humanized mice |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| FCM | 1:250 | Hu |
Background
CD25, also known as IL2RA, is the alpha chain of the interleukin-2 receptor, a heterotrimeric protein complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). It is a membranous glycoprotein primarily expressed on activated T lymphocytes and plays a crucial role in immune homeostasis by interacting with interleukin-2, a pleiotropic cytokine. CD25 is involved in the proliferation of T and B lymphocytes and is essential for the assembly of the high-affinity interleukin-2 receptor. In addition to its role in the immune system, CD25 is also expressed on certain non-immune cells and has been identified as a potential therapeutic target in cancers.
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