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    Intestines Tissue Digestion Solution

    Intestines Tissue Digestion Solution

    Catalog Number: abs9749 Brand: Absin
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    $214 USD
    $214 USD
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    Product Details

    Product Specification

    Usage (1) It is recommended to store the tissue after sampling at 2-8℃, quickly transfer it to a clean laboratory for tissue processing and stem cell separation, take photos and register the information.
    (2) Prepare several petri dishes and add 4℃ pre-cooled primary buffer (or PBS) for later use.
    (3) Disinfect the sample tube, place the tissue in a petri dish, wash it three times with primary buffer (or PBS), remove impurities, and cut the tissue into a volume of about 1~3mm with ophthalmic scissors or scalpels.3Tissue blocks. (It is recommended to add 1.5ml EP tube to shear the tissue)
    (4) Add 5ml of primary intestinal tissue digestion solution to the shredded tissue block and shake for 15-25min at 4℃ for digestion (observe the tissue digestion at any time during the digestion process).
    Note: If the amount of tissue is too small or the biopsy sample is digested with 1ml of primary tissue digest in a 1.5ml EP tube.
    (5) Take a small amount of digestive fluid and observe it under a microscope. After observing a large number of cell clusters or individual cells below 70 μm under the microscope (Figure 1-2), add 3 times the volume of primary buffer (or PBS) to stop the digestion.
    Description

    Human normal intestinal primary tissue digestive fluid can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is suitable for digestive dissociation of human normal intestine in vitro primary culture.
    General Notes The digestive juice of normal intestinal tissue contains nutrients that maintain cell activity and a mixture of various biological enzymes, avoiding repeated freezing and thawing more than twice.
    Storage Temp. Store at -20°C with a shelf life of 1 year.