WB result of Integrin beta 4 Recombinant Rabbit mAb
Primary antibody: Integrin beta 4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 202 kDa
Observed MW: 250 kDa
Integrin beta 4 Recombinant Rabbit mAb (S-1625-170)
Integrin beta 4 Recombinant Rabbit mAb (S-1625-170)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Integrin beta 4 |
| Synonyms | Integrin beta-4; CD104; Itgb4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | A2A863 |
| Clone Number | S-1625-170 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms |
| Positive Sample | mouse spleen, mouse pancreas |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:200 | Ms |
| IF | 1:100 | Ms |
Background
Integrin beta 4 (ITGB4) is a key transmembrane receptor that primarily forms heterodimers with integrin alpha 6 to mediate cell adhesion and signaling, playing crucial roles in maintaining the integrity of epithelial tissues. It is predominantly expressed in epithelial cells and localized at the basement membrane zone, where it contributes to the formation and stabilization of hemidesmosomes, specialized structures that anchor cells to the extracellular matrix. ITGB4 is involved in various biological processes, including cell migration, proliferation, and survival, and its dysregulation has been implicated in several diseases, such as junctional epidermolysis bullosa, a genetic disorder characterized by severe skin blistering. Additionally, ITGB4 has been found to promote tumorigenesis and cancer cell invasion by interacting with other signaling pathways, such as receptor tyrosine kinases, and facilitating epithelial-mesenchymal transition in various cancers.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse colon. Anti- Integrin beta 4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse spleen. Anti- Integrin beta 4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
