WB result of C-Peptide Rabbit mAb
Primary antibody: C-Peptide Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse pancreas lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 9.4, 12 kDa
Observed MW: 12, 13 kDa
Insulin Recombinant Rabbit mAb (S-638-19)
Insulin Recombinant Rabbit mAb (S-638-19)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | INS |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P01308 |
| Clone Number | S-638-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Cz, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:2000 |
Background
Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the INS gene. It is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Proinsulin is cleaved into insulin and C-peptide. The two are stored in the secretory granules of the pancreatic β-cells and eventually released together in equimolar amounts. C-peptide has an important role in facilitating the correct folding of insulin and formation of its disulfide bridges.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
