WB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: NCI-H69 whole cell lysate 20 µg
Lane 3: Y79 whole cell lysate 20 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
INSM1 Recombinant Rabbit mAb,PBS Only (SDT-3165-72)
INSM1 Recombinant Rabbit mAb,PBS Only (SDT-3165-72)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | INSM1 |
| Synonyms | Insulinoma-associated protein 1; Zinc finger protein IA-1; IA1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q01101 |
| Clone Number | SDT-3165-72 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | NCI-H69, Y79, β-TC-6, PC-12 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu |
Background
INSM1 (Insulinoma-associated 1) is a 58 kDa zinc-finger transcription factor transiently expressed during embryogenesis in neuroendocrine tissues, pancreas and nervous system, where it drives differentiation by repressing progenitor genes such as Ascl1 and NeuroD/β2 while promoting cell-cycle exit, and later in life its re-expression in neuroendocrine tumors sustains oncogenesis through feed-forward loops that stabilize MYC proteins and activate PI3K/AKT signaling.
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Picture
Western Blot
WB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: β-TC-6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
WB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
