WB result of INPP4B Recombinant Rabbit mAb
Primary antibody: INPP4B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A204 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Negative control: HepG2 whole cell lysate; HeLa whole cell lysate; A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 110 kDa
This blot was developed with high sensitivity substrate
INPP4B Recombinant Rabbit mAb (S-3210-62)
INPP4B Recombinant Rabbit mAb (S-3210-62)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | INPP4B |
| Synonyms | Inositol polyphosphate 4-phosphatase type II; Type II inositol 3,4-bisphosphate 4-phosphatase |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | O15327 |
| Clone Number | S-3210-62 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A204, MCF7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:100-1:250 | Hu |
Background
INPP4B (inositol polyphosphate 4-phosphatase type II) is a lipid phosphatase that selectively dephosphorylates PI(3,4)P₂ to PI(3)P, thereby acting as a brake on the PI3K/AKT signaling cascade by limiting AKT plasma-membrane recruitment and activation; through this mechanism it functions context-dependently as either a tumor suppressor—restraining proliferation, survival and chemoresistance in breast, prostate, gastric and nasopharyngeal cancers—or, paradoxically, as an oncogenic facilitator via SGK3 activation in subsets of melanoma, colon cancer and AML, while in male germ cells it safeguards DNA integrity and mitochondrial function during spermiogenesis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-INPP4B antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-INPP4B antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-INPP4B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-INPP4B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
