WB result of iNOS Recombinant Rabbit mAb
Primary antibody: iNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 100 ng/ml LPS for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 131 kDa
Observed MW: 128 kDa
iNOS Recombinant Rabbit mAb (S-1701-43)
iNOS Recombinant Rabbit mAb (S-1701-43)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | iNOS |
| Synonyms | Nitric oxide synthase, inducible; Inducible NO synthase (Inducible NOS; iNOS); Macrophage NOS (MAC-NOS); NOS type II; Peptidyl-cysteine S-nitrosylase NOS2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P29477 |
| Clone Number | S-1701-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | RAW264.7 treated with LPS |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Inducible nitric oxide synthase (iNOS) is one of three key enzymes generating nitric oxide (NO) from the amino acid l-arginine. iNOS-derived NO plays an important role in numerous physiological (e.g. blood pressure regulation, wound repair and host defence mechanisms) and pathophysiological (inflammation, infection, neoplastic diseases, liver cirrhosis, diabetes) conditions. iNOS is the synthase isoform most commonly associated with malignant disease.
Picture
Picture
Western Blot
