WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A204 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: Daudi whole cell lysate 20 µg
Low expression control: A204 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 40 kDa
INI-1/SMARCB1 Recombinant Rabbit mAb (S-4068-21)
INI-1/SMARCB1 Recombinant Rabbit mAb (S-4068-21)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | INI-1/SMARCB1 |
| Synonyms | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47 (BAF47); Integrase interactor 1 protein; SNF5 homolog (hSNF5); BAF47; INI1; SNF5L1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q12824 |
| Clone Number | S-4068-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | K562, HeLa, Jurkat, 293T, Daudi, NIH/3T3, Neuro-2a, RAW264.7, mouse brain, mouse spleen, PC-12, rat brain, rat spleen, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SMARCB1, also known as INI1 or BAF47, is a core component of the human SWI/SNF chromatin remodeling complex, which regulates gene transcriptional activity by altering chromatin structure. As a 47-kilodalton protein composed of 385 amino acids and localized in the nucleus, SMARCB1 can bind to DNA and plays critical roles in various life processes, including cell proliferation, differentiation, and tumor suppression. The protein is best known for its function as a tumor suppressor, and mutations or loss of its expression are closely associated with the development of multiple aggressive tumors. For instance, biallelic inactivation of SMARCB1 is a key molecular event leading to rhabdoid tumors—highly malignant neoplasms that predominantly affect infants and young children—and its germline mutations also underlie the rhabdoid tumor predisposition syndrome (RTPS1). Furthermore, variations in the SMARCB1 gene have also been linked to schwannomatosis (a benign nerve sheath tumor disorder) and Coffin-Siris syndrome (a hereditary developmental disorder), demonstrating the diversity of disease spectrums resulting from its functional abnormalities.
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Western Blot
WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: RAW264.7 whole cell lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Lane 5: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 40 kDa
WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 44 kDa
WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 44 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- INI-1/SMARCB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
