WB result of Inhibin alpha Recombinant Rabbit mAb
Primary antibody: Inhibin alpha Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: mouse testis lysate 20 µg
Lane 2: mouse ovary lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
Inhibin alpha Recombinant Rabbit mAb (SDT-3212-24)
Inhibin alpha Recombinant Rabbit mAb (SDT-3212-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Inhibin alpha |
| Synonyms | Inhibin alpha chain; INHA |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P05111 |
| Clone Number | SDT-3212-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse testis, mouse ovary, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:100 | Hu |
Background
Inhibin alpha is a 366-residue glycoprotein that dimerizes to form the common α-subunit of the inhibin family; it is synthesized primarily in Sertoli cells of the testis and granulosa cells of the ovary, where it partners with either a βA or βB chain to create inhibin A or inhibin B, both of which act as potent negative-feedback regulators of pituitary FSH secretion by antagonizing activin signaling through competition for the type II activin receptor, thereby governing gametogenesis and steroidogenesis; beyond reproduction, inhibin alpha is a sensitive serum tumor marker for granulosa-cell and Sertoli-cell neoplasms, and its expression is controlled by gonadotropins, local growth factors, and epigenetic mechanisms, making it a key node in the hypothalamic–pituitary–gonadal axis.
Picture
Picture
Western Blot
WB result of Inhibin alpha Recombinant Rabbit mAb
Primary antibody: Inhibin alpha Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian steroid cell tumor. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human testis. Anti-Inhibin alpha chain antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human kidney. Anti-Inhibin alpha chain antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
