IMP3 Recombinant Rabbit mAb (S-6196)

Catalog Number: S0B60533 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	IMP3
Location	Nucleus
Accession	Q9NV31
Clone Number	S-6196
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Mk
Positive Sample	HeLa, HEK-293, BxPC-3, Hep G2, HT-29, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Mk
IHC-P	1:1000	Hu

Background

IMP3 (insulin-like growth factor 2 mRNA-binding protein 3, gene name IGF2BP3) is an oncofetal RNA-binding protein that is highly expressed during embryonic development and significantly downregulated after birth. It belongs to the IMP family, and its structure contains two N-terminal RNA recognition motifs (RRMs) and four C-terminal KH domains. As a reader of m6A modification, IMP3 regulates the stability, localization, and translational efficiency of transcripts by recognizing N6-methyladenosine-modified mRNAs, thereby exerting critical regulatory functions at the post-transcriptional level. IMP3 is aberrantly overexpressed in various malignant tumors, where it promotes cell proliferation, invasion, and metastasis by stabilizing oncogenic mRNAs such as MYC and CD44 or by activating IGF-2 translation, and it is closely associated with chemotherapy resistance and poor prognosis.

Picture

Western Blot

WB result of IMP3 Recombinant Rabbit mAb
Primary antibody: IMP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: Hep G2 whole cell lysate 20 µg
Lane 5: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 69 kDa

WB result of IMP3 Recombinant Rabbit mAb
Primary antibody: IMP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 69 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-IMP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-IMP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-IMP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-IMP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-IMP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.