2μg (R: reducing condition, N: non-reducing condition).
IL10RA/CD210 Fc Chimera Protein, Cynomolgus
IL10RA/CD210 Fc Chimera Protein, Cynomolgus
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | CD210 antigen, CDw210a, HIL-10R, IBD28, IL-10 R alpha, IL-10 receptor subunit alpha, IL10R alpha, IL-10R subunit 1, IL-10R1, IL10RA, IL-10Ra, IL10RIL-10R subunit alpha |
| Accession | XP_005579838.2 |
| Amino Acid Sequence | His22-Asn235, with C-terminal Human IgG1 Fc HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGTGRWNSISNCSQALSYDLTAVTLDLYRSNGYRARVRAVDGSRHSNWTVTNTRFSLDEVTLTVGSVKLEIHNGFILGKIQPPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVNHENFSLLTSGEVGEFCVQVKPSVTSRTNKGMWSKEECVSLTRQYFTVTNIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Expression System | HEK293 |
| Molecular Weight | 72-90kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 2.Manzanillo, P. et al. (2015) Trends Immunol. 36:471. 3.Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641. 4.Mannino, M.H. et al. (2015) Cancer Lett. 367:103. 5.Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. |
Background
Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 51 kDa transmembrane glycoprotein member of the class II cytokine receptor family. IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Interleukin-10 receptor subunit alpha (IL-10 R alpha) is a cell surface receptor for the cytokine IL-10 that participates in IL-10 mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3.
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SDS-PAGE
SPR
Protein A Chip captured IL10RA/CD210 Fc Chimera, Cynomolgus (Cat. No. UA010643), can bind IL-10 Protein, Cynomolgus (Cat. No. UA040260) with an affinity constant of 0.56nM as determined in SPR assay.
