IL-6 Protein, Human
IL-6 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL-6, BSF2, HSF, IFNB2, IL-6, Interleukin-6 |
| Location | IL-6 |
| Accession | P05231 |
| Amino Acid Sequence | Val30-Met212 |
| Expression System | E.coli |
| Molecular Weight | 21kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 100mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1、Heinrich P C. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20. 2、Rose-John S. et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-624. 3、Dinh W. et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58. |
Background
Interleukin-6 (IL-6) is a member of the pro-inflammatory cytokine family, induces the expression of a variety of proteins responsible for acute inflammation, and plays an important role in the proliferation and differentiation of cells in humans. Interleukin 6 was originally identified as a T cell-derived lymphokine that induces final maturation of B cells into antibody-producing cells. Recombinant human IL-6 acts on B cells activated with Staphylococcus aureus Cowan I or pokeweed mitogen (PWM) to induce immunoglobulin M (IgM), IgG and IgA production, but not on resting B cells. Anti-IL-6 antibody was found to inhibit PWM-induced Ig production, indicating that IL-6 is one of the essential factors in PWM-induced Ig production. Furthermore, IL-6 was shown to augment the primary and secondary anti-SRBC (sheep red blood cell) antibody production in mice in vivo.
Picture
Picture
Bioactivity
SDS-PAGE
ELISA
Immobilized IL-6, Human (Cat. No. UA040052) at 5.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448) with EC50 of 3.04-3.92 ng/mL.
Immobilized IL-6 Protein, Human (Cat. No. UA040052) at 2.0μg/mL (100μL/well) can bind Biotinylated IL-6 R alpha/CD126 Fc&Avi Tag Protein, Human (Cat. No. UA011222) with EC50 of 8.02-11.36ng/mL.
Immobilized Human IL-6 (Cat. No. UA040052) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-6R alpha/CD126 Avi&His Tag Protein, Human (Cat. No. UA011248) with EC50 of 78.37-140.2ng/mL.
SPR
Protein A Chip captured IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448), can bind IL-6, Human (Cat. No. UA040052) with an affinity constant of 8.51nM as determined in SPR assay.
