Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte), treated overnight with 500ng/ml LPS (Red) or untreated (Green), labeling IL-6 at 1/2000 dilution (0.1 μg) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
IL-6 Mouse mAb (S-1038-139)
IL-6 Mouse mAb (S-1038-139)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | IL-6 |
| Synonyms | Interleukin-6; B-cell stimulatory factor 2 (BSF-2); CTL differentiation factor (CDF); Hybridoma growth factor; Interferon beta-2 (IFN-beta-2); IL6; IFNB2 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P05231 |
| Clone Number | S-1038-139 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:2000 | Hu |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
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