Measured by its ability to induce STAT3 phosphorylation by U-87 cells in response to Recombinant Mouse IL-31 His Tag in the presence of 20ng/mL Recombinant Mouse IFN-γ. The EC50 for this effect is less than 30ng/ml.
IL-31 Protein, Mouse
IL-31 Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-31; IL-31; |
| Accession | Q6EAL8 |
| Amino Acid Sequence | Thr24-Cys163, with C terminal His Tag. |
| Expression System | HEK293 |
| Molecular Weight | 20-40 kDa (Reducing) |
| Purity | >95% by SDS-PAGE; >90% by HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Nat Immunol. 2004 Jul;5(7):752-60. Epub 2004 Jun 6. |
Background
Interleukin-31 (IL-31) is a cytokine that belongs to the IL-6 cytokine family and is primarily produced by activated T helper type 2 (Th2) cells. IL-31 signals through a heterodimeric receptor complex composed of IL-31 receptor A (IL-31RA) and oncostatin M receptor (OSMR), leading to the activation of downstream signaling pathways such as STAT1 and STAT3. IL-31 is involved in various inflammatory and immune responses, and has been implicated in the pathogenesis of allergic diseases, skin disorders, and chronic inflammatory conditions. In mouse models, IL-31 has been shown to play a role in pruritus (itching) and dermatitis, highlighting its importance in mediating inflammatory responses in the skin.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>90% as determined by RP-HPLC.
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-31 is immobilized 2.5µg/mL (100µL/well), Recombinant Mouse IL-31RA Fc chimera binds with an EC50 of 0.02-0.081μg/ml.
