Measured in a cell proliferation assay using Murine M-NFS-60 cells. The EC50 for this effect is 0.11-23 pg/mL.
IL-3 His Tag Protein, Mouse
IL-3 His Tag Protein, Mouse
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$145 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-3 |
| Accession | P01586 |
| Amino Acid Sequence | Ala27-Cys166 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 20-35kDa (Reducing) |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Okada S, Nakauchi H, Nagayoshi K, Nakamura M, Miura Y, Suda T. Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors. Exp Hematol. 1992 Jun;20(5):546-51. PMID: 1350248. |
Background
Mouse IL-3 (interleukin-3), also known as multi-colony-stimulating factor, is a small glycoprotein secreted mainly by activated T cells and other cells. It is a short-chain cytokine of the four-helix bundle family and signals through a receptor composed of IL-3Rα (CD123) and the common βc chain (CD131). It promotes the proliferation, differentiation, and survival of multipotent hematopoietic progenitors, particularly expanding mast cells, basophils, eosinophils, and some myeloid cells, and is involved in allergic inflammation, parasite immunity, and sepsis. Clinically, mouse IL-3 is mainly used in models of hematopoiesis, allergy, and inflammatory diseases and in target validation of the IL-3/IL-3R axis; because of species specificity relative to human IL-3, its direct clinical value is limited, but it provides a translational basis for related biomarkers and targeted therapies.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N:non-reducing condition).
SEC-HPLC
The purity of IL-3 His Tag Protein, Mouse is more than 95% determined by SEC-HPLC.
SPR
IL-3 Rα/CD123 Fc Chimera, Mouse (Cat. No. UA010122) captured on Protein A Biosensor, can bind IL-3, Mouse (Cat. No. UA016236) with an affinity constant of 1.28μM as determined in SPR assay.
