Measured in a cell proliferation assay using CTLL2 cells, the EC50 for this effect is less than 0.2 ng/ml.
IL-2(Cys145Ser) Protein, Human
IL-2(Cys145Ser) Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL-2(Cys145Ser), IL-2 C145S |
| Accession | P60568 |
| Amino Acid Sequence | Ala21-Thr153(Cys145Ser) |
| Expression System | E.coli |
| Molecular Weight | 15kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Pol, J. G. , Caudana, P. , Paillet, J. , Piaggio, E. , & Kroemer, G. . (2020). Effects of interleukin-2 in immunostimulation and immunosuppression. The Journal of Experimental Medicine(1), 217. 2.Kolios, A. G., Tsokos, G. C., & Klatzmann, D. (2021). Interleukin-2 and regulatory T cells in rheumatic diseases. Nature Reviews Rheumatology, 17(12), 749-766. 3.Zhang, Y., & Su, J. (2023). Interleukin-2 family cytokines: An overview of genes, expression, signaling and functional roles in teleost. Developmental & Comparative Immunology, 104645. |
Background
IL-2(Cys145Ser) is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. It has been engineered to contain the serine for cysteine substitution at amino acid position 145. IL-2(Cys145Ser) has similar characteristics and functions to IL-2, which is a leukocytotrophic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells, and plays a vital role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity. Recombinant IL-2 is widely used in cell culture for the expansion of T cells.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
