Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells.The ED50 for this effect is 0.589-0.672 µg/mL in the presence of 2.0 ng/mL of recombinant human IL-15.
IL-2 R beta/CD122 His Tag Protein, Human
IL-2 R beta/CD122 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL2RB, RP5-1170K4.6, CD122, P70-75 |
| Accession | NP_000869.1 |
| Amino Acid Sequence | Ala27-Asp239 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 35-40kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE&＞90% by HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Keller HR, Kim HK, Jo Y, Gress RE, Hong C, Park JH. The Abundance and Availability of Cytokine Receptor IL-2Rβ (CD122) Constrain the Lymphopenia-Induced Homeostatic Proliferation of Naive CD4 T Cells. J Immunol. 2020 Jun 15;204(12):3227-3235. |
Background
IL-2Rβ (CD122) serves as the common β subunit shared by both the interleukin-2 receptor (IL-2R) and the IL-15 receptor, forming a dimeric intermediate-affinity receptor with IL-2Rγ (CD132) or a trimeric high-affinity receptor with CD25 (IL-2Rα) and CD132, thereby mediating IL-2 and IL-15 signal transduction through the JAK-STAT pathway. It plays a central role in regulating the development, proliferation, survival, and differentiation of lymphocytes such as T cells and NK cells, particularly influencing CD8⁺ T memory cell maintenance, invariant natural killer T (iNKT) cell generation, and thymocyte development. Clinically, CD122 gene mutations can lead to severe immune dysregulation and peripheral tolerance defects, while aberrant expression is closely associated with immune dysfunction in ANCA-associated vasculitis, systemic lupus erythematosus, inflammatory bowel disease, and HBV/HCV infections. In recent years, CD122-biased IL-2 agonists (e.g., NKTR-214/bempegaldesleukin, ANV419) have emerged as a hotspot in cancer immunotherapy, selectively binding the CD122/CD132 dimer to preferentially activate CD8⁺ T cells and NK cells while reducing regulatory T (Treg) cell-mediated immune suppression, thus offering novel precision therapeutic strategies for cancer and autoimmune diseases.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N:non-reducing condition).
SEC-HPLC
The purity of IL-2 R beta/CD122 His Tag Protein, Human is more than 90% determined by SEC-HPLC.
SPR
Anti-His antibody Immobilized on CM5 Chip captured IL-2 R beta/CD122 His Tag Protein, Human (Cat. No. UA016036), can bind IL-2 Protein, Human (Cat. No. UA040108) with an affinity constant of 0.24 μM as determined in SPR assay.
