IL-2 Protein, Human
IL-2 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL-2,interleukin-2 |
| Accession | P60568 |
| Amino Acid Sequence | Ala21-Thr153, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | Theoretical: 17.1kDa Actual: 17,19kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 1*PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells.IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. In the thymus, where T cells mature, it prevents autoimmune diseases by promoting the differentiation of certain immature T cells into regulatory T cells, which suppress other T cells that are otherwise primed to attack normal healthy cells in the body.
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Bioactivity
SDS-PAGE
ELISA
Immobilized human IL-2RA-Fc at 2μg/ml (50μL/well) can bind to IL-2 with a linear range of 0.17ng/ml to 40ng/ml.
Immobilized IL-2 His Tag, Human (Cat. No. UA040057) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 1.23-1.99 ng/ml.
SPR
Protein A Chip captured IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260), can bind IL-2 His Tag, Human (Cat. No. UA040057) with an affinity constant of 15nM as determined in SPR assay.
