Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells. The IC50 for this effect is 12-21 ng/mL in the presence of 20 ng/mL of recombinant human IL-18.
IL-18BP His Tag Protein, Cynomolgus
IL-18BP His Tag Protein, Cynomolgus
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | Interleukin-18-binding protein |
| Accession | A0A2K5UDJ4 |
| Amino Acid Sequence | Thr28-Pro207 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 35-70kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Novick D. IL-18 and IL-18BP: A Unique Dyad in Health and Disease. Int J Mol Sci. 2024 Dec 17;25(24):13505. |
Background
IL-18BP (Interleukin-18 Binding Protein) is a secreted immunoregulatory glycoprotein belonging to the IL-1 receptor family, whose structure contains a single Ig-like domain that binds IL-18 with high affinity (Kd≈400 pM) and blocks its interaction with the IL-18Rα/β receptors, thereby inhibiting NF-κB and AP-1 signaling pathways and downstream IFN-γ production; recombinant IL-18BP His-tagged protein derived from Cynomolgus monkeys holds critical clinical significance in drug development, serving as a natural antagonist for treating NLRC4-related autoinflammatory diseases, rheumatoid arthritis, and other Th1-mediated autoimmune disorders, while its identification as a secreted immune checkpoint has revealed the resistance mechanism of IL-18 immunotherapy, driving the development of next-generation biologics including anti-IL-18BP antibodies and IL-18 mutants, with this protein also serving as a biomarker for radiation injury and a potential target for radioprotective agents.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N:non-reducing condition).
SEC-HPLC
The purity of IL-18BP His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.
ELISA
Immobilized IL-18 Protein, Human (Cat. No. UA040111) at 1.0μg/mL (100μL/well) can bind IL-18BP His Tag Protein, Cynomolgus (Cat. No. UA016066) with EC50 of 4.36-7.07 ng/mL.
