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IL-17 RA&IL-17 RC Heterodimer His&Tag Free Protein, Human

IL-17 RA&IL-17 RC Heterodimer His&Tag Free Protein, Human

Catalog Number: UA016203 Brand: UA BIOSCIENCE
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$215 USD
$215 USD
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Product Details

Product Specification


Species Human
Synonyms IL-17 RA&IL-17 RC, CD217, IL-17RA, IL-17 receptor C, IL-17RL, IL-17RC
Accession Q96F46-1、NP_703190.1
Amino Acid Sequence

Leu 33-Trp 320 (IL-17 RA) with His Tag at the C-Terminus;
Leu 21-His 465(IL-17 RC) with No Tag
Expression System HEK293
Molecular Weight 60-90kDa&100-130kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag, No Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. WILSON S C, CAVENEY N A, YEN M, et al. Organizing structural principles of the IL-17 ligand-receptor axis[J]. Nature, 2022, 609: 622-629.
2. GOEPFERT A, LEHMANN S, BLANK J, et al. Structural analysis reveals that the cytokine IL-17F forms a homodimeric complex with receptor IL-17RC to drive IL-17RA-independent signaling[J]. Immunity, 2020, 52(3): 499-512.e5.
3. GOEPFERT A, LEHMANN S, WERDER J, et al. IL-17-induced dimerization of IL-17RA drives the formation of the IL-17 signalosome to potentiate signaling[J]. Cell Reports, 2022, 41(9): 111751.

Background

IL-17RA and IL-17RC serve as the principal receptor subunits for IL-17 family cytokines, forming a classic heterodimeric signaling complex. Structurally, both receptors contain fibronectin type III domains in their extracellular regions and engage IL-17A/F ligands via a tip-to-tip assembly mode to form ternary complexes, which further organize into 2:2:2 hexameric signalosomes to amplify downstream signaling. Functionally, this receptor complex drives pro-inflammatory responses through the activation of NF-κB and MAPK pathways, playing a pivotal role in the pathogenesis of autoimmune diseases such as psoriasis and ankylosing spondylitis. Clinically, brodalumab (anti-IL-17RA) and anti-IL-17A monoclonal antibodies targeting this axis have been widely applied in the treatment of inflammatory disorders, while small-molecule inhibitors are currently under active development.

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SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

ELISA

Immobilized IL-17A Protein, Human (Cat. No. UA040483) at 2 μg/mL (100 μL/well) can bind IL-17 RA&IL-17 RC Heterodimer His&Tag Free Protein, Human(Cat. No. UA016203) with a linear range of 5.7-7.8ng/mL.

Immobilized IL-17F Protein, Human (Cat. No.UA040308) at 2 μg/mL (100 μL/well) can bind IL-17 RA&IL-17 RC Heterodimer His&Tag Free Protein, Human(Cat. No. UA016203) with a linear range of 27-35ng/mL.