Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.6ng/ml.
IL-12 Protein,Mouse
IL-12 Protein,Mouse
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$264.60 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | IL-12a Protein, Mouse; IL-12b Protein, Mouse; IL-12p35 Protein, Mouse; IL-12p40 Protein, Mouse; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; CLMF p35; p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NKSF1; NK cell stimulatory factor chain 1; interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; p35 Protein, Mouse; p40 Protein, Mouse |
| Accession | P43432 、P43431 |
| Amino Acid Sequence | p40: Met23-Ser335; p35: Arg23-Ala215, with C-terminal His Tag |
| Expression System | CHO |
| Molecular Weight |
P40: 35.8 kDa (Reducing) P35: 22.5 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE and HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880. 2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074. 3. Gearing, D. et al. (1991) Cell 66:9. 4. Merberg, D. et al. (1992) Immunology Today 13:78. 5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143. |
Background
IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized IL-12 Protein, Mouse (Cat. No. UA040218) at 1.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera Protein, Human (Cat. No. UA010956) with EC50 of 0.05-0.15μg/mL.
