Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.
IL-12 Protein, Human
IL-12 Protein, Human
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$270.00 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell; IL12A ; NKSF1 stimulatory factor chain 1 |
| Accession | P29459、P29460 |
| Amino Acid Sequence | p40:Ile23-Ser328 p35:Arg23-Ser219 |
| Expression System | CHO |
| Molecular Weight | P40:34.7kDa (Reducing) ;P35:22.5kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880. 2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074. 3. Gearing, D. et al. (1991) Cell 66:9. 4. Merberg, D. et al. (1992) Immunology Today 13:78. 5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143. |
Background
IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
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Bioactivity
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by HPLC.
ELISA
Immobilized IL-12, Human(Cat. No. UA040200) at 2.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera, Human(Cat. No. UA010956) with EC50 of 0.18-0.23 μg/mL.
