WB result of IL-10 Rabbit mAb
Primary antibody: IL-10 Rabbit mAb at 1/1000 dilution
Lane 1: IL-10 recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20, 37 kDa
IL-10 Recombinant Rabbit mAb (SDT-088-83)
IL-10 Recombinant Rabbit mAb (SDT-088-83)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-10 |
| Synonyms | Interleukin-10, CSIF |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P22301 |
| Clone Number | SDT-088-83 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | null |
| WB | 1:1000 | null |
Background
IL-10 is an anti-inflammatory cytokine that maintains the balance of the immune response, allowing the clearance of infection while minimizing damage to the host. IL-10 can also dampen the harmful immune responses elicited in autoimmunity and allergy. Many different types of cells can produce IL-10, with the major source of IL-10 varying in different tissues or during acute or chronic stages of the same infection.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
