IL-1 beta/IL-1F2 Recombinant Rabbit mAb (S-1517-211)

Catalog Number: S0B60795 Application: WB, IHC-P, ICC Reactivity: Hu, Ms Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	IL-1 beta
Synonyms	Interleukin-1 beta; Catabolin; IL1F2; IL1B
Immunogen	Recombinant Protein
Location	Cytoplasm, Secreted, Lysosome
Accession	P01584
Clone Number	S-1517-211
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms
IHC-P	1:500	Hu
ICC	1:500	Ms

Background

Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is produced by various cell types, including macrophages and monocytes, in response to infections or tissue injury. IL-1β helps to initiate and amplify the inflammatory response by promoting the expression of other inflammatory mediators and recruiting immune cells to the site of infection or damage. It also has effects on a wide range of physiological processes, such as fever induction, modulation of the hypothalamic-pituitary-adrenal axis, and regulation of metabolism. Dysregulation of IL-1β has been implicated in numerous chronic inflammatory diseases, like rheumatoid arthritis and inflammatory bowel disease, making it an important target for therapeutic interventions.

Picture

Western Blot

WB result of IL-1 beta/IL-1F2 Recombinant Rabbit mAb
Primary antibody: IL-1 beta/IL-1F2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Human IL-1 beta Protein, His tag lysate 1 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 25 kDa

WB result of IL-1 beta/IL-1F2 Recombinant Rabbit mAb
Primary antibody: IL-1 beta/IL-1F2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Raw 264.7 whole cell lysate 20 μg
Lane 2: RAW264.7 were stimulated with LPS (100 ng/mL) for 6 hours, with BFA (300 ng/mL) added during the last 3 hours of stimulation whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 36 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-IL-2 beta/IL-1F2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-IL-2 beta/IL-1F2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IL-2 beta/IL-1F2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC analysis of RAW264.7 cells treated with LPS (100ng/ml, 6hr) and BFA (300ng/ml, last 3hr of stimulation) (top panel) and untreated RAW264.7 cells (below panel). Anti-IL-1 beta/IL-1F2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).