WB result of IKZF1 Recombinant Rabbit mAb
Primary antibody: IKZF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: A20 whole cell lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50-60 kDa
This blot was developed with high sensitivity substrate
IKZF1 Recombinant Rabbit mAb (S-4176-17)
IKZF1 Recombinant Rabbit mAb (S-4176-17)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IKZF1 |
| Synonyms | DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; Ikaros; Lyf1; Zfpn1a1; Znfn1a1; Ikzf1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q03267 |
| Clone Number | S-4176-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse thymus, mouse spleen, A20, rat thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:500 | Ms, Rt |
Background
IKZF1 is a zinc-finger-containing transcription factor belonging to the Ikaros family. It is predominantly expressed in lymphocytes and regulates target gene transcription by recognizing and binding to specific DNA sequences, serving as a critical regulator of hematopoietic and immune system development. It acts as a "master regulator" in lymphocyte differentiation: in the B-cell lineage, it promotes early B-cell development and guides their differentiation into antibody-secreting cells, while also participating in immunoglobulin gene rearrangement; in the T-cell lineage, it controls T-cell maturation, lineage selection, and thymocyte proliferation, while maintaining genomic stability in developing cells. Ikaros functions also extend to the innate immune system, such as regulating the development of dendritic cells and NK cells. Consequently, IKZF1 abnormalities are closely associated with various diseases—its mutations or deletions can lead to hematological malignancies such as B-cell acute lymphoblastic leukemia (B-ALL), while alterations in the IKZF1 gene are also considered risk factors for common variable immunodeficiency (CVID) and autoimmune diseases.
Picture
Picture
Western Blot
WB result of IKZF1 Recombinant Rabbit mAb
Primary antibody: IKZF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Negative control: rat kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50-60 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse colon. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat thymus. Anti-IKZF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
