IKK gamma Recombinant Rabbit mAb (S-1359-118)

Catalog Number: S0B1393 Application: WB, IHC-P, ICC, ICFCM Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	IKK gamma
Synonyms	NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma); NF-kappa-B essential modifier; FIP3; NEMO; IKBKG
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q9Y6K9
Clone Number	S-1359-118
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC, ICFCM
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, PC-3, Jurkat, K562, NIH/3T3, mouse brain, C6, rat brain
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Ms
ICFCM	1:50	Ms

Background

IKK gamma, also known as NEMO (NF-κB essential modulator), is a crucial regulatory subunit of the IκB kinase (IKK) complex. The IKK complex, which also includes the catalytic subunits IKKα and IKKβ, plays a central role in activating the NF-κB signaling pathway. IKK gamma itself does not possess kinase activity, but it is essential for the assembly and activation of the IKK complex. When cells are stimulated by various factors such as cytokines, bacterial products, or viral infections, the IKK complex is activated, leading to the phosphorylation and degradation of IκB proteins. This process releases NF-κB dimers, allowing them to translocate to the nucleus and regulate the expression of numerous genes involved in inflammation, immune responses, and cell survival. Mutations in the gene encoding IKK gamma can lead to severe immunodeficiencies and developmental disorders, highlighting its critical role in maintaining immune function and cellular homeostasis.

Picture

Western Blot

WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Wild-type HeLa whole cell lysate 20 µg
Lane 2: IKK gamma knockout HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 40, 48 kDa

WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa

WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa

WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelled with anti-IKK gamma antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in NIH/3T3 cells. Anti-IKK gamma antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).