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IGFBP-2 Protein, Mouse

IGFBP-2 Protein, Mouse

Catalog Number: UA040533 Brand: UA BIOSCIENCE
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$130 USD
$130 USD
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Product Details

Product Specification


Species Mouse
Synonyms Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, mIGFBP-2
Accession P47877
Amino Acid Sequence

Gln35-Gln305 with His Tag at the C-Terminus
Expression System HEK293
Molecular Weight

38-40 kDa (Reducing)
Purity >95% by SDS-PAGE & HPLC.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, 1mM EDTA, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Cell Metab. 2010 Jan;11(1):11-22.
Cytokine Growth Factor Rev. 2015 Jun;26(3):329-46.

Background

Insulin-like Growth Factor Binding Protein-2 (IGFBP-2) is a 30–36 kDa secreted protein encoded by the Igfbp2 gene, containing 271 amino acids in the mature form after cleavage of a 34-amino acid signal peptide. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 binds both IGF-I and IGF-II with high affinity, exhibiting 2–10 fold higher affinity for IGF-II than for IGF-I. This binding prolongs IGF half-life and modulates ligand-receptor interactions.

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Bioactivity

Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The EC50 for this effect is 12-19 ng/mL in the presence of 14 ng/mL human IGFII.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

RP-HPLC

>95% as determined by RP-HPLC.