2μg (R: reducing condition, N: non-reducing condition).
IGFBP-2 His Tag Protein, Human
IGFBP-2 His Tag Protein, Human
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$160 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2, |
| Accession | P18065 |
| Amino Acid Sequence | Ala36-Gln325, with C-terminal His Tag |
| Expression System | CHO |
| Molecular Weight | 34-36 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3475-80. |
Background
Insulin-like growth factor-binding protein-2 (IGFBP-2) is a 36-kDa cysteine-rich secreted glycoprotein that preferentially binds IGF-II and is highly expressed during embryogenesis, especially in the central nervous system and reproductive tissues. Beyond simply sequestering IGFs, IGFBP-2 possesses intrinsic, IGF-independent activities: it contains an RGD integrin-binding motif, is internalized via a nuclear-localization signal, and can activate PI3K/Akt, NF-κB and β-catenin pathways. Consequently, IGFBP-2 functions as a developmental regulator and, when aberrantly re-activated, as an oncogenic hub that promotes epithelial-to-mesenchymal transition, angiogenesis, stemness and chemo-resistance in multiple solid tumours.
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SDS-PAGE
SEC-HPLC
>95% as determined by SEC-HPLC.
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized IGF-I Protein, Human at 5.0μg/mL (100μL/well) can bind IGFBP-2 Protein, Human His Tag (Cat. No. UA040414) with EC50 of 0.12-0.16ug/ml.
Immobilized IGF-II Protein, Human (Cat. No. UA040162) at 5.0μg/mL (100μL/well) can bind IGFBP-2 Protein, Human His Tag (Cat. No. UA040414) with EC50 of 0.095-0.116ug/ml.
