Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The ED50 for this effect is 44-139 ng/mL in the presence of 6 ng/mL recombinant human IGF-I
IGFBP-1 Protein, Human
IGFBP-1 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12 (PP12) |
| Accession | P08833 |
| Amino Acid Sequence | Ala26Asn259, with C-terminal His tag |
| Expression System | HEK293 |
| Molecular Weight | 35-38 kDa (Reducing) |
| Purity | >95% by Bis-Tris PAGE and HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Trends Endocrinol Metab. 2009 May;20(4):153-62. |
Background
IGFBP-1 is a secreted protein encoded by the IGFBP1 gene located on chromosome 7 in humans. It is a member of the insulin-like growth factor binding protein family, which consists of six high-affinity binding proteins (IGFBP-1 to -6).
Human IGFBP-1 has evolved from being viewed as a passive IGF carrier to being recognized as a dynamic, multifunctional protein with significant IGF-independent biological activities. Its primary research utility lies in its role as a sensitive biomarker for hepatic insulin sensitivity and pregnancy-related conditions. Industrially, it is harnessed for diagnostic immunoassays and explored as a novel therapeutic target. Future research is likely to further dissect its tissue-specific roles and translational potential in metabolic, bone, and neoplastic diseases.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized IGFBP-1 Protein, Human (Cat. No. UA040519) at 2.0μg/mL (100μL/well) can bind IGF-I Protein, Human (Cat. No. UA040520) with EC50 of 11.93-16.50ng/mL.
