IFIH1 Recombinant Rabbit mAb (S-2413-49)

Catalog Number: S0B60497 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	IFIH1
Synonyms	Interferon-induced helicase C domain-containing protein 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa (CADM-140 autoantigen); Helicase with 2 CARD domains (Helicard); Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5 (MDA-5); Murabutide down-regulated protein; MDA5 s; RH116
Immunogen	Synthetic Peptide
Location	Cytoplasm, Mitochondrion
Accession	Q9BYX4
Clone Number	S-2413-49
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Raji, Daudi, T-47D, MDA-MB-231
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500-1:2000	Hu

Background

IFIH1 (Interferon Induced Helicase C Domain Containing Protein 1), also known as MDA5 (Melanoma Differentiation-Associated Gene 5), is a key intracellular retinoic acid-inducible gene I-like receptor (RLR) that specifically recognizes double-stranded RNA (dsRNA) produced during viral replication in the cytoplasm, serving as a sentinel in antiviral innate immunity. Upon viral invasion, IFIH1 binds dsRNA via its helicase domain, subsequently recruiting the downstream adaptor protein MAVS and activating TBK1/IKKε kinases, which then phosphorylate IRF3 and IRF7 to induce the production of type I interferons (such as IFN-α/β) and pro-inflammatory cytokines, launching a broad antiviral immune response. However, dysregulation of IFIH1 function can lead to immune imbalance: its gain-of-function mutations (such as p.A946T) cause hyperactivation of the type I interferon pathway, leading the body to misrecognize self-RNA as viral components, thereby triggering rare autoinflammatory diseases such as Aicardi-Goutières syndrome and Singleton-Merten syndrome; conversely, certain polymorphic variants (such as rs1990760) have been shown to be significantly associated with the risk of common autoimmune diseases including type 1 diabetes and systemic lupus erythematosus, suggesting that IFIH1 plays a delicate regulatory role in maintaining immune tolerance while activating antiviral defenses. Therefore, IFIH1 has become an important drug target for the treatment of autoinflammatory diseases and viral infections.

Picture

Western Blot

WB result of IFIH1 Recombinant Rabbit mAb
Primary antibody: IFIH1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated THP-1 whole cell lysate 20 μg
Lane 2: THP-1 treated with 1 μg/mL LPS for 24 hours whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 117 kDa
Observed MW: 135 kDa

WB result of IFIH1 Recombinant Rabbit mAb
Primary antibody: IFIH1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: Raji whole cell lysate 20 μg
Lane 2: Daudi whole cell lysate 20 μg
Lane 3: T-47D whole cell lysate 20 μg
Lane 4: MDA-MB-231 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 117 kDa
Observed MW: 135 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-IFIH1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-IFIH1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-IFIH1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-IFIH1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-IFIH1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.