WB result of ICAM2/CD102 Rabbit pAb
Primary antibody: ICAM2/CD102 Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 60 kDa
ICAM2/CD102 Rabbit Polyclonal Antibody
ICAM2/CD102 Rabbit Polyclonal Antibody
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ICAM2/CD102 |
| Synonyms | Intercellular adhesion molecule 2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P13598 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Jurkat, HUVEC |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
ICAM2 (Intercellular Adhesion Molecule 2, also known as CD102) is an important member of the ICAM family of cell adhesion molecules within the immunoglobulin superfamily. It is primarily expressed on the surface of endothelial cells, lymphocytes, and certain immune cells, playing key regulatory roles in leukocyte homing, transendothelial migration, and immune synapse formation. The extracellular region of this protein consists of two immunoglobulin-like C2-type domains, which bind to integrin receptors (mainly LFA-1, i.e., CD11a/CD18) and Mac-1 (i.e., CD11b/CD18), while its short cytoplasmic tail connects indirectly to the actin cytoskeleton through ezrin-radixin-moesin (ERM) proteins to support the stability of adhesive structures. In contrast to the widely inducible expression of ICAM1, ICAM2 is constitutively expressed at relatively high levels on resting endothelial cells, primarily mediating rapid initial adhesion and rolling capture of lymphocytes, and promoting stable adhesion and transendothelial migration of leukocytes in coordination with chemokine signals. Furthermore, ICAM2 is involved in regulating T cell receptor signaling, modulating dendritic cell migration, and maintaining blood-brain barrier integrity. Pathophysiological studies have shown that dysregulated expression of ICAM2 is closely associated with various inflammation-related diseases, including autoimmune encephalomyelitis, asthma, atherosclerosis, and dysregulation of tumor immune surveillance, thereby positioning it as a potential intervention target in anti-inflammatory and anti-tumor immunotherapy.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-ICAM2/CD102 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ICAM2/CD102 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ICAM2/CD102 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ICAM2/CD102 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ICAM2/CD102 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
