• IAA ELISA Kit
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IAA ELISA Kit

IAA ELISA Kit

Catalog Number: abs554106 Application: ELISA Reactivity: Other Conjugation:
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$429 USD
$429 USD
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Product Details

Product Specification

protein IAA
Species Reactivity General purpose
Theory This kit adopts the principle of competition law. The microplate was coated with purified IAA to prepare a solid phase carrier, and the sample or standard product and biotin-labeled antibody were sequentially added to the microwells coated with IAA, and streptomycin-HRP was added after the reaction. After thorough washing, color was developed with the substrate TMB. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a negative correlation between the depth of color and the IAA in the sample. The sample concentration was calculated by measuring the absorbance (OD value) with a microplate reader at a wavelength of 450 nm.
Source General purpose
Synonym IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Detection Type Recombinant or native IAA can be detected and does not cross-react with other related proteins.
Composition

Chinese Name

96T

Preservation conditions

Enzyme labeled plate (detachable)

12 Strip × 8 Hole

4°C/-20°C

Lyophilized Standard

2

4°C/-20°C

Standard & Sample dilution

20 mL

4°C/-20°C

Biotin conjugate ( 100× ）

60 μL

4°C/-20°C

Biotin conjugate dilution

10 mL

4°C/-20°C

concentrate HRP Enzyme conjugate ( 100× ）

120 μL

4°C/-20°C

Enzyme conjugate dilution

12 mL

4°C/-20°C

Concentrated wash ( 25× ）

20 mL

4°C/-20°C

Chromogenic substrate solution ( TMB ）

10 mL

4°C/-20°C( Protected from light)

Reaction stop solution

6 mL

4°C/-20°C

Sealing film

2

normal temperature

General Notes

1. If the entire kit is stored at-20 °C, please place the kit at 4 °C the night before the experiment.

2. Salt precipitation will occur when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve.

3. The newly opened wells of the enzyme labeled plate may contain a little water-like substance, which is a normal phenomenon and will not have any impact on the experimental results.

4. This kit is intended for laboratory research and development use only, not for human or animal use.

5. Reagents should be treated as hazardous substances and should be handled carefully and properly disposed of.

6. Gloves, lab coats and protective glasses should always be worn to avoid skin and eye contact with terminating solution and TMB. In case of accidental contact, please wash thoroughly with water.
Storage Temp. Unopened kit, stored at 4 °C, shelf life 6 months.
Test Range 3.13-200 ng/mL; Sensitivity: 0.89 ng/mL