WB result of Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb
Primary antibody: Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 57 kDa
Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb (S-2143-20)
Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb (S-2143-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Hyaluronidase PH20/SPAM1 |
| Synonyms | Hyaluronidase PH-20; Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20; HYAL3; PH20 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P38567 |
| Clone Number | S-2143-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human testis, mouse testis, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
Hyaluronidase PH20, also known as SPAM1 (Sperm Adhesion Molecule 1), is a multifunctional glycosyl hydrolase enzyme belonging to the glycosyl hydrolase 56 family. It is a GPI-anchored protein expressed in the testis and found on the surface of mammalian sperm and the inner acrosomal membrane. PH20/SPAM1 is unique for its hyaluronidase activity at neutral pH, which is essential for sperm to penetrate the hyaluronic acid-rich cumulus cell layer surrounding the oocyte during fertilization. Additionally, it plays roles in zona pellucida binding and calcium signaling associated with acrosomal exocytosis. This enzyme is also involved in sperm maturation and has potential applications in reproductive medicine and contraception.
Picture
Picture
Western Blot
WB result of Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb
Primary antibody: Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
