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Human VEGF ELISA Kit

Human VEGF ELISA Kit

Catalog Number: abs510008 Application: ELISA Reactivity: Human Conjugation:
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$450 USD
$450 USD
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Product Details

Product Specification

Species Reactivity Human
Theory This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human VEGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference corrected wavelength of 540 nm or 570 nm).
Synonym MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGF, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Composition Please use within the expiration date of the kit
form Specifications Shelf life of diluted or redissolved reagent after unpacking
Human VEGF Microplate 1 piece Unused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant
Human VEGF Standard 2 sticks After dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days
Human VEGF detection antibody 1 stick After dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days
40 × SA-HRP 1 stick 40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage
10 × Buffer for concentration and dilution 1 vial After opening, it can be stored at 2-8 °C for 30 days
Chromogenic liquid 1 vial
Stop liquid 1 vial
20 × concentrated wash buffer 1 vial
Sealing film 3 sheets Stored at room temperature, to avoid contamination, not reusable
Background Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor, is an effective regulator of angiogenesis and angiogenesis in fetus and adults. Vascular endothelial growth factor belongs to the PDGF family, a family of proteins characterized by the presence of eight conserved cystine residues at the structure of the cystine knot and the dimer bound by antiparallel disulfide bonds. After alternative cleavage and sequence length of amino acids, humans express different isoforms, including: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189 and VEGF206, among others. Among them, VEGF165 is the highest expression isoform, followed by VEGF121 and VEGF189. With the exception of VEGF121, the other isoforms contain a basic heparin-binding region and do not diffuse freely. Human VEGF165 has 88% homology to the corresponding protein amino acid sequences of mouse and rat. VEGF is expressed in various cells and tissues, including skeletal muscle cells and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34 + stem cells, endothelial cells, fibroblasts, vascular smooth muscle cells, and the like. The expression of VEGF is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatin M and tumor necrosis factor alpha. The expression level of VEGF is also different during development and in adults.
The dimer of VEGF binds to two related tyrosine kinase receptors, namely VEGFR1 (also called Flt-1) and VEGFR2 (Flk-1/KDR). VEGF can induce both homodimerization and autophosphorylation of the latter. These receptors have seven extracellular immunoglobulin domains and one intracellular separate tyrosine receptor domain. Both vascular endothelial cells and some other non-endothelial cells express VEGF receptors. Although VEGF has the highest affinity with VEGFR1, VEGFR2 is the main factor regulating angiogenesis of VEGF. VEGF165 also binds to the semaporin receptor, Neuropilin-1, thereby promoting complex formation with VEGFR2.
VEGF is known for its involvement in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, and thus regulates the density and volume of blood vessels. But it doesn't work on the pattern of vascularization. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemokine. Postpartum, VEGF maintains the integrity of vascular endothelial cells and is an effective mitogen for large/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and the female reproductive cycle. In diseased tissues, VEGF promotes vascular permeability. Therefore, VEGF is involved in the metastatic process of tumors through extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control tumor angiogenesis induced by VEGF. The level of VEGF circulating in the body is related to the extent of autoimmune diseases (such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, etc.).
General Notes 1. Please use the kit within the validity period.
2. The components of different kits and different batch kits cannot be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.
4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.
Storage Temp. Kit unopened, stored at 2-8 °C.
Test Range 31.3pg/mL-2000pg/mL

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