Schematic diagram of Human Treg Panel Kit.
Human Treg Panel Kit (CD3/CD4/CD8/CD25/FOXP3)
Human Treg Panel Kit (CD3/CD4/CD8/CD25/FOXP3)
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$670 USD
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$670 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This fivecolor panel is designed for detecting regulatory T cells (Tregs) in human peripheral blood or other biological samples. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T cells and cytotoxic T cells subpopulations. CD25 is a marker often used to detect Tregs, and FOXP3 was included as a specific transcription factor for Tregs; it is important to note that FOXP3 is an intracellular marker and requires fixation and permeabilization. Finally, Tregs were identified as CD25+FOXP3+ cells within the helper T cells gate. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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FC
Human PBMCs were stained using the Human Treg Panel Kit consisting of anti-Human CD3 APC-Cy7, anti- Human CD4 FITC, anti- Human CD8 PerCP-Cy5.5, anti- Human CD25 PE and anti- Human FOXP3 Alexa Fluor® 647 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the lymphocyte population.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD3 APC-Cy7-A vs SSC-A dot plot show the T cells with the live cell gate applied.
4. Create a CD4 FITC-A vs CD8 PerCP-Cy5.5-A dot plot with the CD3+ gate applied.
5. Create a CD25 PE -A vs FOXP3 Alexa Fluor® 647-A dot plot and apply the CD4+ gate. Create a gate to encompass CD25+ FOXP3+ cells (Treg cells).
