Human PBMCs were stained using the Human Tissue-Resident T Cells Kit consisting of anti-human CD3 APC-Cy7, anti-human CD8 Pacific Blue, anti-human CD4 FITC, anti-human CD69 PE, anti-human CD103 PerCP-Cy5.5 and anti-human CD62L APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD4 FITC-A vs CD8 Pacific Blue-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
4. Create a CD62L APC-A vs SSC-A dot and apply the CD3+ gate.
5. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L+ gate. Create gates to encompass CD69- CD103- cells (Central memory T cells, TCM)
6. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L- gate. Create gates to encompass CD69+CD103+ cells (Canonical tissue-resident memory T cells, TRM), CD69+CD103- cells (Non-epithelial tissue-resident T cells)
Human Tissue-Resident T Cells Kit
Human Tissue-Resident T Cells Kit
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This six‑color panel is designed for characterizing T‑cell subsets, with a focus on tissue‑resident memory T cells, in human peripheral blood or other biological samples. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. CD62L is a marker often used to distinguish central memory T cells from effector and tissue‑resident subsets. Finally, the combined expression of CD69,CD103 and CD62L can identify canonical tissue‑resident memory T cells, non‑epithelial tissue‑resident T cells and central memory T cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
Picture
Picture
FC
PHA stimulated Human PBMCs were stained using the Human Tissue-Resident T Cells Kit consisting of anti-human CD3 APC-Cy7, anti-human CD8 Pacific Blue, anti-human CD4 FITC, anti-human CD69 PE, anti-human CD103 PerCP-Cy5.5 and anti-human CD62L APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD4 FITC-A vs CD8 Pacific Blue-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
4. Create a CD62L APC-A vs SSC-A dot and apply the CD3+ gate.
5. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L+ gate. Create gates to encompass CD69- CD103- cells (Central memory T cells, TCM)
6. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L- gate. Create gates to encompass CD69+CD103+ cells (Canonical tissue-resident memory T cells, TRM), CD69+CD103- cells (Non-epithelial tissue-resident T cells)
DLS
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