Schematic diagram of Human TH1/TH2/TH17 Cell Panel Kit.
Human TH1/TH2/TH17 Cell Panel Kit
Human TH1/TH2/TH17 Cell Panel Kit
Price:
Regular price
$670 USD
Regular price
Sale price
$670 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Th1, Th2, Th17 polarized human PBMC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This five‑color panel is designed for detecting T helper subsets, including Th1, Th2, and Th17 cells, human peripheral blood or other biological samples. CD3 was included as a general T‑cell marker, and CD4 was included to identify helper T cells (CD4+). Intracellular cytokine staining for IFN‑γ, IL‑4, and IL‑17A was used to identify Th1 (IFN‑γ+), Th2 (IL‑4+), and Th17 (IL‑17A+) subsets; it is important to note that these cytokines are intracellular markers and require fixation and permeabilization before staining. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
Picture
Picture
FC
Th1, Th2, Th17 polarized human PBMCs were stained using the Human TH1/TH2/TH17 Cell Panel Kit consisting of anti-human CD3 APC-Cy7, anti-human CD4 PE-Cy7, anti-human IFN-γ APC, anti-human IL-17A FITC, and anti-human IL-4 Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 583 (S0B88803) vs SSC-A dot plot with the human leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD3 APC-Cy7-A vs CD4 PE-Cy7-A dot plot show the CD4+ T cells with the live cell gate applied.
4. Create an IL-17A FITC -A vs IFN-γ APC -A dot plot and apply the CD4+ gate applied. Create a gate to encompass IL-17A+ IFN-γ- cells (Th17 cells), IL-17A+ IFN-γ+ cells (Th1/Th17 cells), IL-17A- IFN-γ+ cells (Th1 cells).
5. Create an IL-4 Pacific Blue -A vs SSC-A dot plot and apply the CD4+ gate applied. Create a gate to encompass IL-4+ cells (Th2 cells).
