Schematic diagram of Human TE/TN/TCM/TEM Panel Kit.
Human TE/TN/TCM/TEM Panel Kit
Human TE/TN/TCM/TEM Panel Kit
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$670 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This five‑color panel is designed for characterizing T‑cell subpopulations, including naïve T cells, central memory T cells, effector memory T cells, and effector T cells in human peripheral blood. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. Finally, the expression pattern of CCR7 and CD45RA staining on T cells can distinguish distinct memory/effector subsets: naïve T cells (TN), central memory T cells (TCM), effector memory T cells (TEM), and effector T cells (TE). The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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FC
Human PBMCs were stained using the Human TE/TN/TCM/TEM Panel Kit consisting of anti-mouse CD3 APC-Cy7, anti-mouse CD8 PerCP-CY5.5, anti-mouse CD4 FITC, anti-mouse CD45RA PE-Cy7, and anti-mouse CCR7 APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD3 APC-Cy7-A vs SSC-A dot plot show the T cells with the CD45+ gate applied.
4. Create a CD4 FITC-A vs CD8 PerCP-Cy5.5-A dot plot with the CD3+ gate applied.
5. Create a CCR7 APC-A vs CD45RA PE-Cy7-A dot plot and apply the CD8+CD4- gate and the CD8-CD4+ gate. Create gates to encompass CCR7+ CD45RA- cells (Central memory T cells, Tcm), CCR7+ CD45RA+ cells (Naive T cells, Tn), CCR7- CD45RA- cells (Effector memory T cells, Tem), CCR7- CD45RA+ cells (Teff).
