1 1

Human T Cell Panel Kit

Human T Cell Panel Kit

Catalog Number: S0N0017 Application: FCM Reactivity: Hu Conjugation: Brand: Starter
Share:
Price:
$585 USD
$585 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Application FCM
Reactivity Hu
Positive Sample Human peripheral blood cells
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Background

This four‑color panel is designed for detecting T‑cell subpopulations in human peripheral blood or other biological samples. CD45 serves as a pan-leukocyte marker. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.

Picture

FC

Schematic diagram of Human T Cell Panel Kit

Human peripheral blood cells were stained using the Human T Cell Panel consisting of anti-human CD45 APC-Cy7, anti-human CD3 Alexa Fluor® 488, anti-human CD8 Alexa Fluor® 488 and anti-human CD4 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the human peripheral blood cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the human peripheral blood cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 Alexa Fluor® 488-A vs SSC-A dot plot show the T cells and apply the CD45+ gate.
4. Create a CD8 Alexa Fluor® 647-A vs CD4 PE-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.