Human PBMCs were stained using the Human T+B+NK+Monocyte panel Kit consisting of anti-human CD3 APC-Cy7, anti-human CD4 PerCP-Cy5.5, anti-human CD8 Pacific Blue, anti-human CD19 FITC, anti-human CD56 PE and anti-human CD14 Alexa Fluor® 700 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD14 Alexa Fluor® 700-A vs SSC-A dot show the monocytes with the Fixable Viability Dye 545- gate applied.
4. Create a CD3 APC-Cy7-A vs CD56 PE-A dot plot and apply the CD14- gate. Create gates to encompass CD3+ CD56+ cells (NK-T cells), CD3- CD56+ cells (NK cells)
5. Create a CD3 APC-Cy7-A vs CD19 FITC-A dot plot and apply the CD14- gate. Create gates to encompass CD3+ CD19- cells (T cells), CD3- CD19+ cells (B cells)
6. Create a CD4 PerCP-Cy5.5-A vs CD8 Pacific Blue-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD56/CD14)
Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD56/CD14)
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This six‑color panel is designed for detecting T cells, B cells, natural killer (NK) cells, NKT cells, and monocytes in human peripheral blood. CD3 was included as a general T-cell marker, and CD4 and CD8 were included to identify helper T-cell (CD4+) and cytotoxic T-cell (CD8+) subpopulations. CD56 is a marker often used to detect NK and NKT cells; it is important to note that NKT cells also express the T-cell markers CD3 and CD4 or CD8. B cells were identified by CD19, which is a general B-cell marker. Finally, the expression pattern of CD14 and CD16 staining on monocytes can identify monocyte subpopulations, namely, classical, intermediate, and nonclassical monocytes.The panel is compatible with most three-laser flow cytometers. lf desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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