Human PBMCs were stained using the Human T+B+NK+Monocyte panel Kit consisting of anti-human CD3 APC-Cy7, anti-human CD8 PerCP-Cy5.5, anti-human CD4 FITC, anti-human CD19 PE, anti-human CD16 PE-Cy7 and anti-human CD14 Alexa Fluor® 700 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD14 Alexa Fluor® 700-A vs SSC-A dot and apply the Fixable Viability Dye 452- gate.
4. Create a CD3 APC-Cy7-A vs CD19 PE-A dot plot and apply the CD14- gate. Create gates to encompass CD3+CD19- cells (T cells), CD3-CD19+ cells (B cells)
5. Create a CD4 FITC-A vs CD8 PerCP-Cy5.5-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
6. Create a CD3 APC-Cy7-A vs CD16 PE-Cy7-A dot plot and apply the CD14- gate. Create gates to encompass CD3+CD16- cells (T cells), CD3-CD16+cells (NK cells)
7. Create a CD14 Alexa Fluor® 700-A vs CD16 PE-Cy7-A dot plot and apply the CD14+ gate. Create gates to encompass CD14+CD16- cells (Classical monocytes), CD14+CD16+ cells (Intermediate monocytes)
Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD16/CD14)
Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD16/CD14)
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This six‑color panel is designed for detecting T cells, B cells, NK cells, and monocytes in human peripheral blood or other biological samples. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. CD19 was included as a general B‑cell marker to identify B cells . CD16 is a marker often used to detect NK cells. Finally, the expression pattern of CD14 and CD16 staining on monocytes can identify monocyte subpopulations, namely, classical, intermediate and nonclassical monocytes. monocytes. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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