Human sFlt-1 OneStep ELISA Kit

Catalog Number: S0C3249 Reactivity: Hu Conjugation: Brand: Starter

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$500 USD

Size: 1x96T

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Product Details

Product Specification

Antigen	sFlt-1
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 6.7%； Inter-assay: 9.5%
Sample type	Serum; Heparin Plasma; Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	66.08 pg/mL
Range	312.5 pg/mL – 20,000 pg/mL
Recovery	Serum: 116% Heparin Plasma: 110% Cell Culture Supernatant: 106%
Assay time	60 minutes
Species reactivity	Hu

Background

Human sFlt-1 (soluble Fms-like tyrosine kinase-1; sVEGFR-1) binds to VEGF-A, VEGF-B, and PlGF and functions as a decoy receptor to inhibit vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is secreted by placental trophoblasts, endothelial cells, renal mesangial cells, and some hematopoietic stem cells. It competes with VEGFR2 for VEGF binding and thereby modulates VEGFR2-mediated endothelial cell proliferation and vessel branching. sFlt-1 release from monocyte/macrophage lineage cells can suppress their migration and alter the secretion of growth factors and cytokines.

Picture

ELISA

Standard Curve
Example of Human sFlt-1 standard curve in Assay Diluent HA4. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Human sFlt-1 was evaluated in activated samples spiked with concentrations spanning the entire assay range.

Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Human sFlt-1 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.