Human PD-L1/B7-H1 ELISA Kit

Detection principle:

This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-humanPD-L1/B7-H1Antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, thePD-L1/B7-H1Binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin was added(Streptavidin-HRP)。 After washing, a chromogenic substrate is added to protect the color from light. Add a stop solution to stop the reaction, and at450 nmwavelength(Reference correction wavelength540nmOr570nm)The absorbance value was determined.

Standard curve plot

Background:

Programmed death factor ligand1 (PD-L1), also known asB7-H1AndCD274, is an approximately65 kDaTransmembrane glycoproteins, belonging toB7Family of immunomodulatory molecules.PD-L1Expressed in immune cells activated by inflammation, including macrophages,TCells andBCells, keratinocytes, endothelial cells and intestinal epithelial cells, as well as various cancer cells and melanoma.PD-L1Can be withPD-1AndTCellsB7-1/CD80Binding, inhibitionTActivation and proliferation of cells, inducing activatedTApoptosis. It works by promotingTCell inactivation and regulationTCell development, which plays a role in the process of immune tolerance.PD-L1Beneficial to the anti-inflammatory processIL-10AndIL-22Development of dendritic cells, inhibition ofTh17Development of cells. In cancer,PD-L1Able to resistTCell-mediated lysis, enhanced the ability of epithelial cells to transition to mesenchyma, enhancedTh22The tumorigenic function of cells. In plasma of cancer patients and cerebrospinal fluid of glioma patients, its solublePD-L1It will rise somewhat. It can be released by mature dendritic cells and retains bindingPD-1And inductionTCapacity of apoptosis.